Tag Archives: Protocols

Bacteriophages: Methods and Protocols: Molecular and Applied Aspects

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Martha R. J. Clokie, Andrew M. Kropinski, "Bacteriophages: Methods and Protocols: Molecular and Applied Aspects"
Humana Press | December 15, 2008 | ISBN: 1603275649 | PDF | 320 pages | 11.2 MB
Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 2: Molecular and Applied Aspects examines a multitude of topics, including the bacteriophage genomics, metagenomics, transcriptomics, and proteomics, along with applied bacteriophage biology. Written in the highly successful Methods in Molecular Biology series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.

Bacteriophages: Methods and Protocols: Molecular and Applied Aspects
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Astronomy Hacks: Tips & Tools for Observing the Night Sky

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Astronomy Hacks: Tips & Tools for Observing the Night Sky by Robert Bruce Thompson, Barbara Fritchman Thompson
English | 2005 | ISBN: 0596100604 | 412 pages | PDF | 80 MB

Astronomy Hacks: Tips & Tools for Observing the Night Sky
Why use the traditional approach to study the stars when you can turn computers, handheld devices, and telescopes into out-of-this-world stargazing tools? Whether you're a first timer or an advanced hobbyist, you'll find Astronomy Hacks both useful and fun. From upgrading your optical finder to photographing stars, this book is the perfect cosmic companion.This handy field guide covers the basics of observing, and what you need to know about tweaking, tuning, adjusting, and tricking out a 'scope. Expect priceless tips and tools for using a Dobsonian Telescope, the large-aperture telescope you can inexpensively build in your garage. Get advice on protocols involved with using electronics including in dark places without ruining the party.Astronomy Hacks begins the space exploration by getting you set up with the right equipment for observing and admiring the stars in an urban setting. Along for the trip are first rate tips for making most of observations. The hacks show you how to:

Dark-Adapt Your Notebook Computer
Choose the Best Binocular
Clean Your Eyepieces and Lenses Safely
Upgrade Your Optical Finder
Photograph the Stars with Basic Equipment
The O'Reilly Hacks series has reclaimed the term "hacking" to mean innovating, unearthing, and creating shortcuts, gizmos, and gears. With these hacks, you don't dream it-you do it–and Astronomy Hacks brings space dreams to life. The book is essential for anyone who wants to get the most out of an evening under the stars and have memorable celestial adventures.
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RNA Bioinformatics (Methods in Molecular Biology)

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RNA Bioinformatics (Methods in Molecular Biology) by Ernesto Picardi
English | 2015 | ISBN: 1493922904 | 415 pages | PDF | 19 MB

RNA Bioinformatics (Methods in Molecular Biology)
This volume provides an overview of RNA bioinformatics methodologies, including basic strategies to predict secondary and tertiary structures, and novel algorithms based on massive RNA sequencing. Interest in RNA bioinformatics has rapidly increased thanks to the recent high-throughput sequencing technologies allowing scientists to investigate complete transcriptomes at single nucleotide resolution. Adopting advanced computational technics, scientists are now able to conduct more in-depth studies and present them to you in this book. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible bioinformatics protocols, and key tips to avoid known pitfalls.

Authoritative and practical, RNA Bioinformatics seeks to aid scientists in the further study of bioinformatics and computational biology of RNA.
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RNA Remodeling Proteins: Methods and Protocols

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Marc Boudvillain, "RNA Remodeling Proteins: Methods and Protocols"
English | ISBN: 1493922130 | 2015 | 486 pages | PDF | 12 MB

RNA Remodeling Proteins: Methods and Protocols
This volume provides readers with current methods to study RNA remodeling proteins. The methods, ranging from basic to complex, help the scientific community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters where readers will find procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, seeks to aid scientists in the further study of this ever evolving field of proteins and mechanisms.
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Evidence-Based Geriatric Nursing Protocols for Best Practice: Fourth Edition

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Marie Boltz, Elizabeth Capezuti, Terry T. Fulmer, DeAnne Zwicker, Ardis O'Meara, "Evidence-Based Geriatric Nursing Protocols for Best Practice: Fourth Edition"
English | ISBN: 0826171281 | 2011 | PDF | 744 pages | 6,5 MB

Evidence-Based Geriatric Nursing Protocols for Best Practice: Fourth Edition
"The evidence-based protocols are designed as a primary reference and are useful, substantive, and timelyThe broader contributions of useful format and succinct review of the evidence make it likely that this text will continue to be the leading resource in nursing education and practice."–The Gerontologist

"Now more than ever, nurses are called upon to lead efforts to embed evidence-based practice in daily operations. As the IOM report states, 'nurses have key roles to play as team members and leaders for a reformed and better-integrated, patient-centered health care system.' The process of implementing sweeping change in health care will likely take years; however, nurses must start pragmatically and focus on these critically important protocols that have demonstrated improved outcomes for older adults. Simply stated, 'Pick this book up and use it.'"

From the Foreword, Susan L. Carlson, MSN, APRN, ACNS-BC, GNP-BC, FNGNA
President, National Gerontological Nursing Association

"As a gerontological clinical educator/research nurse, I will often use this as a reference. The format and the content are good, and the explanations of how to best use the evidence simplify the process of sifting through mountains of information to figure the best practice." Score: 97, 5 Stars. –Doody's

One of the premier reference books for geriatric nurses in hospital, long-term, and community settings, this 4th edition has been thoroughly updated to provide the most current, evidence-based protocols for care of common clinical conditions and issues in elderly patients. Designed to improve the quality, outcomes, and cost-effectiveness of health care, these guidelines are the result of collaboration between leading practitioners and educators in geriatric nursing and New York University College of Nursing.

Protocols for each clinical condition have been developed by experts in that particular area, and most have been systematically tested by over 300 participating hospitals in "Nurses Improving Care for Health System Elders" (NICHE). Evidence is derived from all levels of care, including community, primary, and long-term care. A systematic method in compliance with the AGREE appraisal process was used to rate the levels of evidence for each protocol. Protocols are organized in a consistent format for ease of use, and each includes an overview, evidence-based assessment and intervention strategies, and an illustrative case study with discussion. Additionally, each protocol is embedded within chapter text, which provides the context and detailed evidence for the protocol. Each chapter contains resources for further study.
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Plant Gene Transfer and Expression Protocols (Methods in Molecular Biology) by Heddwyn Jones

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Plant Gene Transfer and Expression Protocols (Methods in Molecular Biology) by Heddwyn Jones
English | Sep 27, 1995 | ISBN: 089603321X | 458 Pages | PDF | 30 MB
The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes-a key to the under­ standing of the biochemical basis of growth and

Plant Gene Transfer and Expression Protocols (Methods in Molecular Biology) by Heddwyn Jones
development in plants.
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High-Throughput Phenotyping in Plants: Methods and Protocols (Methods in Molecular Biology)

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Jennifer Normanly, "High-Throughput Phenotyping in Plants: Methods and Protocols (Methods in Molecular Biology)"
English | ISBN: 1617799947 | 2012 | PDF | 376 pages | 6,9 MB

High-Throughput Phenotyping in Plants: Methods and Protocols (Methods in Molecular Biology)
Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid. Visual screens and morphometric analysis have yielded a plethora of interesting mutants and traits that have provided insight into complex regulatory pathways, and yet many genes within any given plant genome remain undefined. The premise underlying High Throughput Phenotyping in Plants: Methods and Protocols is that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. The methods described in this volume can be generally classified as quantitative profiling of cellular components, ranging from ions to small molecule metabolites and nuclear DNA, or image capture that ranges in resolution from chlorophyll fluorescence from leaves and time-lapse images of seedling shoots and roots to individual plants within a population at a field site. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, High Throughput Phenotyping in Plants: Methods and Protocols serves as an invaluable guide to plant researchers and all scientists who wish to better understand plant growth and development.
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Protein Nanotechnology: Protocols, Instrumentation, and Applications, Second Edition (Methods in Molecular Biology)

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Protein Nanotechnology: Protocols, Instrumentation, and Applications, Second Edition (Methods in Molecular Biology) by Juliet A. Gerrard
English | ISBN: 162703353X | 2013 | PDF | 350 pages | 7,8 MB

Protein Nanotechnology: Protocols, Instrumentation, and Applications, Second Edition (Methods in Molecular Biology)
Since the first edition of Protein Nanotechnology Protocols Instruments and Applications the intersection of protein science and nanotechnology has become an exciting frontier in interdisciplinary sciences. The second edition of Protein Nanotechnology Protocols Instruments and Applications expands upon the previous editions with current, detailed chapters that provide examples of proteins which are now being harnessed for a wide range of applications, some more developed than others. This book also delves into engineering proteins and an overview of the sorts of tools that are now readily available to manipulate the structure and function of proteins, both rationally and using methods inspired by evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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